05 August 2025 1 4K Report

Dear professors,

Could you kindly recommend a primer for lncRNA HULC that has been previously used and produces sharp, specific bands?

More Seyede Samane Nabavian's questions See All
Why in some wavelengths there are "overflows" in my Carbon dot UV-Vis Spectroscopy results(Emission segment)?

Hi everyone I have got this test in a fixed excitation and various emissions. In some wavelengths there are overflows which make the resulted analyze different from other carbon dots. Is it normal...

18 July 2024 4,244 2 View

Is there a difference between spanning junction primer and divergent primer for designing primers for circular RNAs?

Dear professors I want to design a primer for circ_0000745 and I want to know the difference between spanning junction primer and divergent primer. Which of these two is more efficient?

08 January 2024 2,624 0 View

How do they design primers for circ_0000745?

Dear professors I want to work on circ_0000745 in AML , please guide and advise how to design forward and reverse primers for circ_0000745?

02 January 2024 7,395 2 View

How do they design primers for circular RNAs?

I want to order pair of primers for a circular RNA , what is your suggestion? To design a specific primer for circular RNAs, are there any special conditions compared to the normal primer design?

25 November 2023 6,056 0 View

Direct using of biochar particles in seed germination test pertidish?

Dear colleagues, I would like to design a research plan and use biochar particles in wheat seeds germination tests under salinity stress. I am unsure if the biochar granules or particles can be...

02 August 2023 7,445 4 View

Can we combine fire pumps & Fire water tank for High Velocity Water Spray system, hydrant system and Sprinkler system.?

Can we combine fire pumps for High Velocity Water Spray system, hydrant system and Sprinkler system.? I Want to design water base fire protection system for Substation & Oil type transformer...

03 October 2021 829 1 View

What will be the effect of coating on metal surface.?

If a iron metal rod is kept outside in ambient air (temp condition extreme low and high -10 to 50 degree) and another iron with some paint (normal oil paints)/ zinc coat kept . What will be the...

03 October 2021 8,040 4 View

What is the problem with the xrd method for detecting anatase, rutile and brookite phases?

what is the best way to identify these phases in the sample?

12 January 2020 8,688 6 View

Oscillatory behavior in the spectrum taken by a high-resolution spectrometer?

In my experiment, I need to track the amplitude change of the peaks in my spectrum. I have two spectrometers, one with 0.5nm resolution and the other one with 0.05nm. In the spectrum taken by...

17 February 2019 4,114 13 View

Which step in RNA extraction by TRIZOL is the cause of getting phenol/guanine (260/230 less than 1) contamination?

I have two question, I extracted RNA from cancerous cell line by TRIZOL/Chloroform, the concentration and 260/280 absorbance is very well, but the 260/230 is not. First: Which step can cause...

30 October 2018 9,047 0 View

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Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

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RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

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Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

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RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

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