Using lab mice from cancer experiments for another experiment?

Suppose that you have lab mice that you used for human breast cancer research (e.g. growing human breast cancer cells in the mice abdomen). Once you remove the tumor from the mice, can you use...

09 January 2021 559 3 View

How can i determinate Chondroitin Sulfate Sodium from cream or gel formulation using HPLC?

I am working for Chondroitin Sulfate Sodium determination from gel and cream formulations but when i read my samples in HPLC i see %65 area compared with EP standart. How do you work with...

17 September 2019 6,960 3 View

Is the Hill Coefficient between a monovalent DNA binding site and a monovalent repressor always equal to 1?

And is it also correct when there are multiple independent copies on a single DNA molecule. Also, how binding kinetics differs between a titration based experiment and in vivo when there is a...

08 March 2017 2,126 1 View

Is it possible that there is no significant difference in serum insulin, SOD and LDH level between STZ-induced diabetic rats and healthy rats?

We performed a research regarding duration of diabetes. Diabetes mellitus was induced by injection of STZ (65 mg/kg, i.p). There were two period of diabetes in experimental animals; short term (2...

19 June 2016 2,465 5 View

N2a mouse neuroblastoma cell culture problem?

26 May 2016 7,002 5 View

Which parameter to use in MaxQuant analysis

Hi everyone, I am working on a protein in macrophage cells. My aim is to discover its interacting partners through LC-MS/MS. I have two conditions. One is where the protein stays intact and...

01 January 1970 6,386 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View