Do you know of an in-house protocol for determining lentiviral titer using real-time PCR?

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Aarón Barraza

I suggest first to establish a standard curve for a target gene from your lentivirus. If you have some lentivirus gene cloned, design the primers to amplify a region no more than 150 bp by qPCR, and quantify the amount of plasmid with your lentivirus gene to carry out a standard curve by serial dilutions of this plasmid, to establish a standard curve to determine your lentiviral titer correlating plasmid (sequence amount against amplification cycle threshold).

Luis Quintino

Hi:

Please check the methods section of the following paper where we have described a in house qPCR protocol and feel free to contact me if you have further questions.

http://www.ncbi.nlm.nih.gov/pubmed/23881415

Best Regards,

Luis