Can anyone recommend a protocol for the co-ip of nuclear complexes?

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Daniela Bruennert

http://www.activemotif.com/catalog/25/nuclear-complex-co-ip-kit

I was using this kit to isolate at first the nuclei from my cells and go in a second step for Co-IP, it is working very nice.

Robert Bronstein

Daniela I actually just ordered that very same kit, think it will be compatible with using Protein A/G magnetic beads for the subsequent IP? I have the NEB magnetic beads. Thanks.

I have used both the beads from Active Motif as well as from NEB, and it was both working fine.