Dear all,
I have to administer bromocriptine (BC) to mice to inhibit prolactin release to analyse the role of this hormone in behaviour. BC has a very low solubility in water. People usually do a stock (saturated) solution of BC in pure ethanol and then disolve it in PBS or water and inject it s.c. Since the final concentration of alcohol might be nearly 10%, BC injections are probably very painful, and this may invalidate the results of the ensuing behavioural analysis.
I've thought of using DMSO, in which it is much more soluble. Has anybody experience of drug administration in solutions containing relatively high concentrations of DMSO (about 1%)? Could I use i.p. injections, in that case?
Many thanks
Fernando
We are dissolving it in as a mixture with tartaric acid and just two drops of ethanol. It is not painful at all when injecting!
"Bromocriptine (2-bromo-α-ergocryptine) was mixed with an equal weight of tartaric acid and two drops of 99% ethanol. Thereafter it was dissolved in sterile water and given i.p. once daily"
http://www.ncbi.nlm.nih.gov/pubmed/17157933
Hope this helps.
/Hanna
There is also this possibility:
J Microencapsul. 2002 Jul-Aug;19(4):473-84.
Biodegradable bromocryptine mesylate microspheres prepared by a solvent evaporation technique. I: Evaluation of formulation variables on microspheres characteristics for brain delivery.
Arica B1, Kaş HS, Orman MN, Hincal AA.
Hi!
We used DMSO in the dosage of 1 ml /1000 g i.p. for rats and the animals bore the injections well. There were no changes of behaviour in comparison with intact rats detected at visual observation.
The authors of this work to do likewise:
Docosahexaenoic Acid Reduces ER Stress and Abnormal Protein Accumulation and Improves Neuronal Function Following Traumatic Brain Injury
Gulnaz Begum, Hong Q. Yan, Liaoliao Li, Amneet Singh, C. Edward Dixon, and Dandan Sun
J. Neurosci., Mar 2014; 34: 3743 - 3755. doi: 10.1523/JNEUROSCI.2872-13.2014
I agree with most of the above answers, i also like to add that the researcher can dissolve the proper dose of BK 0.2 ml of DMSO and then inject.
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