Do you know any software to measure Eimeria oocysts by using microscope connected with a computer?
Hi we used DP2-BSW Soft Imaging Soft ware for measuring spore or other particules. this software combined with Olympus BX51 in our lab. May be it can be solution for your problem
Dear Tran,
there are several image analysis systems which are created for different purposes. Each company producing microscopes have its own image analysis software.
One of several software produced by Olympus company is QuickPHOTO MICRO system. This system is basic but is sufficient for your purposes. Nikon, for example, produce more sophisticated NIS-Elements software. Each microscopy sales specialist give you more information
Best regards
Jarda
Dear Cagri and Jarda,
Thank you so much!
I dont know that my computer can use your mention software or not. Can I download this software (Olympus BX51, or Quickphoto micro system) on internet then install in my computer??
Have a nice day!
Tran Nguyen
Unfortunately you have to buy this software and they are relatively expensive. Most of these image analysis systems require a software key.
Jarda
I used a software called ImageJ for evaluation of photos made on the fluorescence microscope.
hı Klaus in ImageJ can we measure something. Because used it but i could not measured any thing this stiuation was related with software or with me?
I used it for quantification of parasites, but did not measure length/width of oocysts... you probably would need to calibrate and constantly use the same camera/microscope settings. Measurement of an area is possible as far as I remember... maybe this helps?
Thank for all of you!! ^-^
So my laboratory dont have much money to buy this software. Just follow the traditional measurement by using microscope ruler
When you say measure do you mean to determine the dimensions? or do you simply want to identify and count?
Dear Mr Jerry Ongerth,
I would like to measure the length, wide in order to identify species. With the support of software, the measurement will be more accurate!
Dear Tran Nguyen
if you can save your image files (or screen copy) and if you can calibrate the image (i.e. 1pixel = xx µm) by using the image of a know length (i.e the microscope ruler), you may open these files with the free open source software GIMP to measure the length and width of the oocyst (or any object) by using tool -> measure. Then you will be able to convert pixels into µm in an excel or libreoffice sheet.
With GIMP you can also add a mark or a number on each object you are measuring so you can make a link between images and measurement data, avoid duplicates and make valuable statistical analyses
You can download GIMP at www.gimp.org
hoping this would be helpful
Dear Maurice Mahieu,
I will try it immediately. Thank you so much! I hope that it works.
Have a nice day!
Tran Nguyen
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