How to dissolve cage-shaped silsesquioxane?

13 January 2021 1,190 3 View

Equipment for immunohistochemistry in free floating sections?

So I have been doing IHC on sections mounted on glass slides for a while. Lots of advantages to this, but one disadvantage is double labeling of proteins in single neurons. For this part of my...

09 December 2019 6,451 2 View

Can a city become more competitive if more demand is added to its main roadway?

Considering that transport is the main component of the logistics system, making domestic production more competitive is basically a strategic decision. How can increased demand for a city's...

20 May 2019 4,193 17 View

What are the consequences of urban dispersion in cities?

I'm reading Alain Bertaud's book - Order without Design: How Markets Shape Cities - and much is discussed about urban planning and, given the circumstances, what decisions should planners make?...

23 March 2019 4,118 9 View

How to remove stuck frit inside HPLC column?

I have stuck frit inside an HPLC column I want to remove, anyone knows how to remove it?

16 February 2019 5,514 6 View

What's the best human macrophage cell line for estimation of the diferential gene expression of S. aureus during a infection celular model?

I need to compare the gene expression of S. aureus during infection celular model.

30 July 2018 1,064 2 View

Absorbance / Emission of qubit BR DNA assay?

Does anyone know the abs/em of qubit's broad range dsdna assay? I have a lot of leftover reagents, and no qubit, but I do have access to a plate reader.

23 April 2018 8,271 2 View

Building RadSeq library with gDNA isolated without RNase treatment. Anyone suspect an issue?

Hi, I'm making RADseq libraries using fish fin clips that have been stored in a chaotropic buffer (4M Guanadinium thiocyanate + detergent, EDTA, TRIS and B-ME). Ideally, I'd like to homogenize...

08 March 2018 3,765 2 View

How do I calculate the absorbance for BBCEAS-like systems?

15 September 2017 6,473 1 View

No title

Hi, Some weeks ago I sent some paraffin blocks and sections (mouse tissues from a very important experiment) to another lab and just today I was informed that the person who received the package...

03 January 2017 6,247 6 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

Hi everyone, I want to lower the pH of microbiological broth, what are the best options?

I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.

03 February 2021 6,959 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

How do you quantify bacterial qPCR data?

Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...

01 February 2021 7,153 4 View