How do I quantify the protein amount in fluorescence-based microplate assay?

I try to read enzymatic activity in tissue homogenates using fluorescence emission in a microplate reader. I hypothesize that a specific treatment can reduce amount of enzyme in tissue and/or...

31 December 2014 6,757 11 View

Can anyone share their experience / literature on the effect of CO2 quality on cell culture?

I noticed that some CO2 suppliers provide different range of puritz (from 99.0% to 99.99%). I was wondering how much CO2 quality may impair or enhance reproducibility of cell culture...

06 July 2014 8,494 9 View

In immunohistochemistry, which serum do you mix to the secondary antibody to reduce aspecific staining?

In immunohistochemistry, many protocols recommend to block the tissue to analyze prior the first antibody incubation with the serum of the animal in which the secondary antibody is raised...

06 July 2014 7,873 21 View

How does one collect blood to avoid red serum?

09 October 2013 7,025 8 View

No title

07 August 2013 6,134 8 View

Effect of high APS concentration in acrylamid gel

I was wondering what would be the effect of too high a concentration of APS in an acrylamid gel. I am also curious to know how people manage their stock of APS (we dilute APS with water at 10%...

31 December 2012 7,747 13 View

What is the importance of HRP-conjugated antibody in sandwich ELISA?

Are antibodies already HRP-conjugated more sensitive than the two steps "antibody + washing + anti species HRP-conjugated"? If yes, kind you suggest any kit to do it?

11 December 2012 6,429 11 View

Can anyone recommend a good source for purchasing plasmids?

I would like to buy plasmids from mouse. My aim is to clone a protein of interest inserting a promoter and a tag (we already choose them and have them in the lab). We would then like to transfect...

09 October 2012 6,664 47 View

handling replicate and repeat measurement in ANOVA

Sorry for this relatively simple question which need a long explanation In a experiment, --> to illustrate: I am counting the number of step that mice need to walk trough a 10 cm long beam I...

08 September 2012 8,134 11 View

Monitoring and quantifying proteins present in nucleus of cell using immunofluorescence histological approach

We are transfecting HEK cells using a vector expressing a protein normally located in the nucleus of cells. By mutating the nuclear localization signal of the protein of interest, we would like to...

08 September 2012 2,419 14 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

How to highlight novelty in your research paper?

I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...

28 February 2021 2,393 8 View

Any suggestions for a website that allows you to create questionnaires and gather unlimited data (Totally free)?

I have found only four of them; www.surveyplanet.com www.online-anket.gen.tr www.surveey.com and of course google forms. Expecting to see your suggestions if there is any. Thank you

28 February 2021 3,237 3 View

What is the value of Conference Review Paper?

How much value does a review paper carry in a higher study aspirant student's profile which is presented in a Peer Reviewed Scopus Indexed conference?

27 February 2021 2,068 3 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Do I need to ask permission to use pictures for my questionnaire?

I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...

23 February 2021 1,631 3 View