Which WB STAT3 and STAT5 antibodies are best?

Hi, I'm currently having problems with Western blots using the following antibodies from Cell Signaling: Phospho-Stat3 (Tyr705) STAT3 (total) Phospho-Stat5 (Tyr694) STAT5 (total) They seem to...

07 August 2019 5,920 5 View

How can I efficiently isolate CD138+ cells from myeloma patients' bone marrow samples?

First, I'm using Ficoll Histopaque centrifugation to obtain mononuclear cells. Then, I'm doing RBC lysis. I observe a drastic loss of CD138+ cells after that (as checked using FACS and two...

11 December 2017 6,561 13 View

Does anyone have suggestions on NGS Whole-exome sequencing - controversial thresholds?

03 April 2015 1,723 7 View

Circulating micro-RNA isolation - which method do you prefer?

I am new to the field of circulating nucleic acid detection. I would like to search for microRNA down/upregulation in blood samples from patients suffering from certain type of cancer. I have...

02 March 2015 9,817 9 View

Which reporter plasmid do you recommend for mutated gene transcription analysis?

I have found a mutation that probably decreases the level of transcription of my gene of interest. I would like to clone a part of the gene into a plasmid that would contain a reporter gene, so...

10 November 2014 3,249 9 View

Can anyone verify whether this is an artifact or an insertion in these Sanger sequencing results?

Not having much experience with sequencing, I would like somebody to look at the attachment. According to the sequence results, there is an insertion between 68G and 70A. That cytosine peak is not...

02 March 2014 3,498 26 View

How can you measure the activity of ONLY extrinsic or ONLY intrinsic pathways in apoptosis?

I would like to measure the activity of FAS/TNFR-dependent pathways and apoptosome-dependent pathways separately using FACS or a fluorometer. I do NOT want to use Western blot. The best solution...

02 March 2014 2,616 21 View

Has anyone experienced very low transfection efficiency using Lipofectamine 2000?

There is a major problem with transfections in our project. I have recently tested Lipofectamine 2000 and a Cyan Fluorescent Protein (CFP) plasmid on CHO adherent cells. According to the protocol,...

01 February 2014 8,241 28 View

H9C2 cardiomyocytes - Lipofectamine 2000 transfection conundrum?

I would like to know if any of you have ever worked with H9C2 cells and successfully transfected them. Alternatively, has anyone worked with any type of cardiomyocytes and performed a successful...

10 November 2013 8,826 8 View

Confocal microscopy novice - can anyone help with fixing and permeabilizing?

We're trying to observe BAG3 expression in H9C2 cardiomyocytes using a primary Alexa Fluor 488nm anti-myc-Tag Ab (the BAG3 is tagged with myc and is expressed from a transfected plasmid). We tried...

10 November 2013 9,722 15 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View