Do you know a good antibody against HBV capsid for western blotting ?

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Ishtiaq Qadri

Abcam has good antibody to HBV Core, lot better than San Cruz.

http://www.abcam.com/hepatitis-b-virus-core-antigen-antibody-c1-ab8637.html

Product nameAnti-Hepatitis B Virus Core Antigen antibody [C1]

See all Hepatitis B Virus Core Antigen primary antibodies

DescriptionMouse monoclonal [C1] to Hepatitis B Virus Core Antigen

SpecificityThis antibody reacts with HBV Core Antigen (Major antigenic determinant, c1). Ab8637 should recognize both the precoreprotein and core protein. It will not recognize the precorprotein under native conditions, because this protein can not self assemble into particles.

Tested applicationsELISA, IHC-Fr, IP, WB, ICC/IFmore details

Species reactivityHas been tested in ADW Hep B subtype.

ImmunogenPurified Hepatitis B Core Antigen

EpitopeAround aa positions 70-80, major, or "c1" determinant

Camille Hallez

Thank you. I saw that Abcam proposes antibodies to HBV core but I didn't know what was the best (because they have 2 or 3 different antibodies) ! So I hoped somebody had already tested them, because technical sheets can be beautiful but far from truth...

Dear Camille we are using Anti-Hepatitis B Virus Core Antigen antibody [C1] (ab20523) and it is working well in WB.

Application: ELISA, EM, ICC/IF, WB

Thanks a lot for your help. Do you have a very specific detection in WB, or is there a background noise ?

Tian Xia

For HBV particle assay, quite similar to WB, we use a Dako anti-HBc antibody. It works. You can found the procedure in this paper.

http://jvi.asm.org/content/81/22/12472.short

Good luck.

Thanks for your answers. I tested the Anti-Hepatitis B Virus Core Antigen antibody [C1] (ab20523) and I have nothing in WB, even if I use Femto ECL or little diutions (I tried 1/50 for example). Actually, I want to detect viral particles produced after transfection of pCayw, and not after infection. I tested the Abcam antibody and 2 Santa Cruz (one against HBc and the second one against pre-S1) and I never detect the virus, whereas I do have viral DNA detected in qPCR (I precipitate the viral particles and then I extract DNA or do a WB). So if one of you has a good solution, I'll take it !

Tian Xia, thanks for your help. I'm gonna test it, I wanted forst when I saw litterature but somebody in the team told me it was not that efficient in WB... Moreover, I can't find informations about its price on the Dako website.