How to extract RNA from brown adipose tissue(BAT)?

30 January 2021 6,610 3 View

Are there any easy way to test the activity of CYP51?

I'm looking for a relative easy way to test the activity of CYP51. Are there any way that I can measure it without using LC-MS or gas chromatography. A simple absorbance or fluorescence test will...

08 October 2020 6,687 3 View

How to extract RNA from pancreas?

RNA extraction from pancreas of ruminants is very difficult due to the existence of abundant RNase. Previously, our research group tried to remove the pancreas from animal immediatley after...

20 September 2020 1,097 2 View

How to get bending moment in Ansys shell181 element?

Hello, I would like to compare the bending moment of a tube with beam and shell element in ansys? The tube has the same dimension in beam and shell element. In beam element, i can get the...

24 August 2020 2,352 1 View

Roche has stopped its Universal Probe primer design service, does anyone know where or how we can design primers to fit Universal Probe Library?

Roche announced the Universal Probe primer design service will be stop in the end of 2020, but the primer design website has already been shutted...

10 February 2020 4,745 2 View

Reusing C18 ziptip while desalting the peptide sample before LC-MS/MS ?

Reusing C18 ziptip while desalting the peptide sample before LC-MS/MS ? Hello everyone I have a question while using ziptip Do you suggest that reuse the zip after one sample binding and...

11 December 2019 7,865 3 View

What reason can affect drug concentration to cell viability in MTT assay ?

I did MTT to find the best drug(for cancer) concentration to do Western blot, as drug concentration got higher cell viability got lower, but at the highest concentration has a peak on cell...

14 August 2019 3,745 5 View

Does mesoporous silica NP dissolve in water?

Hi, guys. Recently, I was doing research on mesoporous silica. I used the traditional ingredient to synthesis it, namely TEOS, CTAB, ammounia aqueous, ethanol and water. To my surprise, when I...

08 July 2019 9,961 5 View

What kind of matrigel for orthotopic injection of breast cancer cells into the mammary fat pad of mice?

I am planning to do orthotopic injection of breast cancer cells (MDA-MB-231 and 4T1 cells) into the mammary fat pad of mice to observe cancer metastasis and tumor growth. In lots of references I...

04 July 2019 6,278 5 View

How to measuring extracellular ATP level?

How to measuring extracellular ATP level in tumor specimens, not the culture cell? Is there are commecial Kits available?

17 November 2018 1,053 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Rearranging abbreviation list in LaTeX ?

Hi, I'm trying to rearrange an abbreviation list for a manuscript such that it would be listed in alphabetical order. Is there a function that does this automatically or is manual my only option?...

18 February 2021 4,870 2 View

If a journal doesn't provide doi after acceptance of the manuscript, is it authentic?

It is related to submission of the manuscript in a Journal and the process of its online availability.

18 February 2021 9,269 5 View

Can anyone provide an advice on running a validation study of a commercially available product in collaboration with the manufacturer?

We are planning to run a study validating a commercially available product (i.e. software) and while we initially planned to do it independently of the manufacturer, they heard about our intention...

17 February 2021 2,295 3 View