Is there a natural conservant to increase shelf life of seaweed liquid extract? Sugarcane molasses could be used?

We are looking for methods to increase lifeshelf of seaweed extracts to be used as liquid fertilizer which can maintain the organic carachteristic of the product. Does anyone has expertise in this...

12 November 2020 1,570 2 View

MiRNA expression and knock-down in nude mice

10 May 2017 8,608 3 View

Is the TP53 gene entrielly deleted in the osteosarcoma cell line Saos-2?

02 February 2017 7,412 1 View

For a small set of genes which would be the best technique to use for gene expression: PCR array, microarray or still some NGS technique?

I have identified by RNA-seq a set of genes which I want to study in more detail using three different cell lines and conditions. This would be about 100 genes or I could expand to 1500. Which...

13 December 2016 1,461 3 View

What are the statistics of success in the use of stem cells in veterinary medicine, specially for dogs? Is there data about it?

What are the cases of success versus non success

10 July 2013 6,456 1 View

Does anyone have comments on the Cytell Flow Cytometer from GE?

I am doing a project and considering asking for funding to buy one.

18 November 2012 7,697 4 View

Please, does anyone in Brazil cultivate a non cancerous bone cell line such as hFOB 1.19?

01 January 1970 2,660 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View