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Questions related from Mozart Marins
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
05 August 2024 366 2 View
It appears in this link below and I tried contact but no answer. Maybe someone has a more direct contact....
05 December 2023 9,950 0 View
I have seen once a video of a machine for planting Kappaphycus using tie tie. I would like to recover the video and contact people who made it.
29 July 2023 8,271 1 View
I am reviewing literature on K. alvarezii UV screen pigments and metabolites and though I find description of Mycosporine-like amino acids (MAAs) in other red seaweeds, I don´t find any...
02 October 2022 5,155 0 View
Please, has anyopne used CheckMateTwo-hybrid, Nanobit or Lumit from PROMEGA or PLA Duo Link from Sigma for studying protein interaction and discovery of drug inhibitor of the interaction?
31 January 2022 5,874 3 View
We are unisng Dual luciferase assays for experiments trying to find interactions between proteins and the the recomendation for using the Dual luciferase assay from Promega is to use luminometers...
09 March 2021 3,037 1 View
We are looking for methods to increase lifeshelf of seaweed extracts to be used as liquid fertilizer which can maintain the organic carachteristic of the product. Does anyone has expertise in this...
13 November 2020 1,813 2 View
Pease, we are new to the field, and text in manuscripts are sometimes confusing. I want to study the effect of overexpression and down expression of a given miRNA in tumor development. Is it...
11 May 2017 8,700 3 View
We intend to screen substances that impact promoter actvity. People usually have a promoter/luciferase gene construct and read luciferase activity with a luminometer. Besides LacZ, CAT and GFP,...
05 February 2017 6,518 2 View
When performing conventional RT-PCR we did not detect TP53 expression, but when performing real time PCR with a Taqman assay for the exon 10-11 boundary, we detected expression. Maybe there´s a...
03 February 2017 7,541 1 View
I have identified by RNA-seq a set of genes which I want to study in more detail using three different cell lines and conditions. This would be about 100 genes or I could expand to 1500. Which...
14 December 2016 1,560 3 View
What are the cases of success versus non success
11 July 2013 6,547 1 View
I am doing a project and considering asking for funding to buy one.
19 November 2012 7,816 4 View
I would like to have as a control for my experiments, a non cancerous cell line of bone origin. Hfob is a an alternative but didn´t find someone who cultivates this cell line in Brasil. Thanks for...
01 January 1970 2,770 3 View