Mozart Marins

14 Questions 10 Answers 0 Followers

Questions related from Mozart Marins

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

05 August 2024 363 2 View

Please have anyone seen or have the contact of the developers of rhis machine: seaweed planting table with tie line inserter?

It appears in this link below and I tried contact but no answer. Maybe someone has a more direct contact....

05 December 2023 9,947 0 View

Mechanization of Kappaphycus using tie-tie method, does anyone knows about it?

I have seen once a video of a machine for planting Kappaphycus using tie tie. I would like to recover the video and contact people who made it.

29 July 2023 8,265 1 View

Are Mycosporine-like amino acids (MAAs) present in Kappaphycus alvarezii?

I am reviewing literature on K. alvarezii UV screen pigments and metabolites and though I find description of Mycosporine-like amino acids (MAAs) in other red seaweeds, I don´t find any...

02 October 2022 5,153 0 View

Which is best Protein Interaction assay?

Please, has anyopne used CheckMateTwo-hybrid, Nanobit or Lumit from PROMEGA or PLA Duo Link from Sigma for studying protein interaction and discovery of drug inhibitor of the interaction?

31 January 2022 5,871 3 View

Dual-Luciferase Reporter Assay System from Prtomega without using injectors? Have anyone tried?

We are unisng Dual luciferase assays for experiments trying to find interactions between proteins and the the recomendation for using the Dual luciferase assay from Promega is to use luminometers...

09 March 2021 3,033 1 View

Is there a natural conservant to increase shelf life of seaweed liquid extract? Sugarcane molasses could be used?

We are looking for methods to increase lifeshelf of seaweed extracts to be used as liquid fertilizer which can maintain the organic carachteristic of the product. Does anyone has expertise in this...

13 November 2020 1,810 2 View

MiRNA expression and knock-down in nude mice

Pease, we are new to the field, and text in manuscripts are sometimes confusing. I want to study the effect of overexpression and down expression of a given miRNA in tumor development. Is it...

11 May 2017 8,696 3 View

Is there a good alternative for luciferase reporter gene which does not need a luminometer?

We intend to screen substances that impact promoter actvity. People usually have a promoter/luciferase gene construct and read luciferase activity with a luminometer. Besides LacZ, CAT and GFP,...

05 February 2017 6,515 2 View

Is the TP53 gene entrielly deleted in the osteosarcoma cell line Saos-2?

When performing conventional RT-PCR we did not detect TP53 expression, but when performing real time PCR with a Taqman assay for the exon 10-11 boundary, we detected expression. Maybe there´s a...

03 February 2017 7,538 1 View

For a small set of genes which would be the best technique to use for gene expression: PCR array, microarray or still some NGS technique?

I have identified by RNA-seq a set of genes which I want to study in more detail using three different cell lines and conditions. This would be about 100 genes or I could expand to 1500. Which...

14 December 2016 1,557 3 View

What are the statistics of success in the use of stem cells in veterinary medicine, specially for dogs? Is there data about it?

What are the cases of success versus non success

11 July 2013 6,545 1 View

Does anyone have comments on the Cytell Flow Cytometer from GE?

I am doing a project and considering asking for funding to buy one.

19 November 2012 7,812 4 View

Please, does anyone in Brazil cultivate a non cancerous bone cell line such as hFOB 1.19?

I would like to have as a control for my experiments, a non cancerous cell line of bone origin. Hfob is a an alternative but didn´t find someone who cultivates this cell line in Brasil. Thanks for...

01 January 1970 2,767 3 View