Do you have any recommendations of a sofware or tool to align peptide fragments obtained by MS/MS?

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References on how to comment a quotation in academic paper?

I need references on how to comment a quotation in academic paper and kinds of comments.

26 October 2023 5,364 1 View

What effect have the use of high antibiotic concentrations in the agrobacterium culture growing on the efficiency T-DNA transfer to the plant cell?

31 August 2023 2,566 2 View

How do I interpret p and q in the corARMA correlation structure?

I understand that p is the autoregessive order and q is the moving average, but I don't know how to interpret what does it means when it is (2,3) or (2,2) for example. Can anyone give me a simple...

09 October 2018 6,443 0 View

Do you have any recomendations for a MALDI-TOF MS instrument?

I am exploring the possibility of buying of a Maldi-Tof MS instrument for my lab. I am familiar only with Bruker Maldi-ToF MS. My main application is characterization of novel peptides so accuracy...

02 March 2018 1,864 6 View

Does anyone have experience ordering custom peptides from the chinese company Synpeptide???

I´m planing to order some custom peptides. Previosly,I ordered peptides from Biomatik which were good but while searching for others options the chinese company Synpetide is offering me a very...

24 May 2017 2,182 3 View

More sequencing is needed to profile functional diversity than phylogenetic diversity, is this true?

There is a commentary in Langille et al., 2013: Predictive functional profiling of microbial communities using 16S r RNA marker gene sequences. "More sequencing is needed to profile functional...

27 March 2017 7,167 3 View

Are tandem repeats of rDNA target for SSR markers?

Ribosomal DNA (rDNA) is tandemly repeated upstream or downstream. Are SSR (ISSR / AFLP) markers capable or targeting multilocus sequences in the ribosomal (rDNA)? If yes, how effective are SSR...

15 February 2017 2,654 1 View

Is C value paradox applied only for genome size, or to genome complexity, as well?

C value paradox describes the lack of relationship between genome size and organismal complexity (Buchanan Biochemistry and Molecular Biology of Plants, 2nd Edition p. 405). I wonder if this...

08 February 2017 9,979 4 View

Could you share your experiences with the Autoflex speed TOF/TOF from Bruker?

Dear colleagues, my lab is considering to acquire the autoflex speed TOF/TOF. Our main application is proteomics but we are also interested in the Biotyper microorganism identification. Could you...

12 January 2017 7,599 2 View

Can i infect BHK-21 with poliovirus strain?

Hi, anyone knows if is possible to infect the cell line BHK21 with poliovirus sabin strain? or with another poliovirus strain?

06 October 2016 5,743 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How can major oxides determined by ICP-MS/ES be normalized to 100% volatile free? How does this connect with LOI?

I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this

05 August 2024 7,902 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View