Hello,
Is there someone that have experience with tamoxifen administration to mice with DMSO as vehicle?
Does anyone know why it is so difficult to find literature about that?
Thanks!
Hi, I am doing three different Multiplex PCR, each with five pairs of primers amplifying regions of different genes. I tested the primers individually first and they all work fine. When i put them...
24 March 2024 4,710 2 View
Dear All, I am trying to run the VUMAT-UMAT interface provided on soilmodel.com (https://soilmodels.com/download/vumat_umatinterface-zip/) with the Sand Hypoplasticity. However, when I run the...
05 September 2023 201 2 View
I am facing a problem with some tridimensional dunes and I wanted to ask you a suggestion. In your opinion, what is the best way to characterize a 3D geometry of dunes. The available data are...
16 November 2022 7,990 3 View
Hello everyone! I need to amplify a specific DNA fragment in order to do Sanger sequencing for the detection of a point mutation in human patients. The fragment is in an exon of my gene of...
25 December 2020 2,026 3 View
My research deals with the Political comunication used by the Feminist parties all around the Europe (in particular in Germany and in the Scandinavian countries). My aim is to demonstrate the...
08 November 2017 6,660 4 View
We are a group of four students taking part in the Virtual Nottingham Consultancy Challenge working alongside Pixelshrink Digital Impact. We are conducting a study into the needs and challenges...
01 January 1970 1,928 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Research on politics administration relation can conduct based on case study method?
01 August 2024 9,557 1 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View