Do you have any information about automated ChIP protocol?

More Mehmet Varol's questions See All

SAP2000 Hinge PMM Surface Not Convex ?

I have modeled bridge pier. I have assigned plastic hinges to circular piles and oblong column. I am getting error only in oblong column hinge as PMM surface is not convex. I am not taking any...

14 May 2024 8,820 0 View

What do you think about using AMBER99SB charge when docking with peptides using Autodock Vina?

I have 1215 peptides between 4 and 13 mer in length. I want to dock them with peptides using Autodock Vina, but I used AMBER99SB instead of Kollman charge to give better and reliable results. I...

11 May 2024 8,014 2 View

How can I improve the efficiency of single gRNA to hybridize with Cas9 to get the RNP complex?

We use synthetic gRNA from IDT but we think have some problems for sgRNA hybridization with Cas9. any suggestion is welcomed!

25 March 2024 1,536 3 View

I am looking for a teammate for an article I will write about gastronomy tourism?

I'm thinking of starting an article about gastronomy tourism soon. For this reason, I need a teammate who can offer different perspectives and produce quality publications and is suitable for...

04 December 2023 8,403 6 View

The Strength of Granules and Agglomerates Author: H. Rumpf Publication Year: 1962 is this resource unavaliable?

I've searched various academic databases and online platforms but have come up short of accessing the full content. If anyone has a copy or knows of a way to access this paper, I would be...

22 October 2023 7,514 0 View

How Lignin, Cellulose, and Hemicellulose is related to biomass combustion fuel properties?

Hello, researchers of biomass. I believe many of you possess invaluable insights on this topic. Beyond basic introductory content, I am keenly interested in sourcing comprehensive resources that...

22 October 2023 4,889 1 View

If a journal is in different quarterlies in two different fields of the WoS classification, which one should we consider?

For example, a journal to which I am considering submitting a manuscript is included in different quartiles (Q2 and Q3) of WoS in both environmental sciences and sustainable science and...

09 October 2023 7,927 4 View

How can i interpret this QSAR graph (pEC50, training set and test test) ?

Hello everyone, I use ChemMaster for QSAR modelling. Firstly, i calculated pEC50 and finded Molecular Weight, rotatable bonds, LogP. Then, with Kennard- Stone Algorithm i divided the datasets...

27 September 2023 5,862 1 View

Two digestions of the same sample give out different results in ICP. Possible reasons?

Dear colleagues, I'm currently working with ICP-OES, and we've been doing twin digestions of the same sample for crosschecking. From time to time, I get significant differences between results...

09 July 2023 5,399 6 View

I am seeking of NZ2 strain ORFV virus. Is there the virus in your labs collection?

Dear Researchers, I need 2 ml of NZ2 strain ORFV virus for my student Ph thesis. Could you send us, buyer pay. My address: Prof.Dr. Mehmet KALE Department of Virology Faculty of Veterinary...

29 May 2023 7,105 6 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View