13 November 2014 1 202 Report

Please help me with mouse Tead4 primers for qPCR. Who have the good working pair?

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What analytical equations are used to calculate nuclear gradients in dft?

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How to remove dirt from diamonds for AFM study?

Hello everybody. I'm trying to study HPHT synthetic diamond plates with low (first nm roughness). When i taking 100×100 μm photo by AFM, you can see a lot of flakes/dirt on them. The question is...

25 October 2023 7,952 4 View

How to freeze atomic positions and set atomic charges when optimizing geometry in pybel?

I'm trying to do something like docking, but for crystals, in the task of transcribing XANES spectra, estimating the random positions of inserted atoms into a fixed crystal structure using a graph...

23 October 2023 3,850 0 View

What type of filter should be used when printing with titanium powder in an SLM printer?

Dear Colleagues! I would like to know your advice about printing with titanium powder in an SLM printer. Will a HEPA filter be enough to filter the gas from residual printing products when working...

20 August 2023 9,837 1 View

How can I automatically get charges of each carbon and nitrogen atom in skeleton of molecule after gaussian calculation?

Hi, I have a list of output files after a Gaussian calculation, and I want to get the charges of carbon and nitrogen atoms in the constant skeleton of these molecules to use them later as QSAR...

17 July 2023 6,438 1 View

What is the place of PLTs in the actual practice of foundation design in the US? What US documents regulate calculation methods?

Hello! I am doing a review on Plate load test in global practice. I have started by looking at foundation design practices in general and I have questions when looking at the US code system and...

25 June 2023 3,166 2 View

Litterature on China-ASEAN sustainable cooperation?

Dear all, cuurntly im a writing a graduation paper on the topic of cooperation between China and ASEAN in terms of sustainable development. I've already found quite a few official reports and...

02 March 2023 7,435 0 View

Tell me what the arrows in the algorithm mean ?

I would like to know the translation, what does the word mean "zoom" (translation needed word. I will be very grateful for the answer!

19 February 2023 1,569 0 View

Google Scholar Preprint Bug - any way to avoid?

The bug when published paper did not appear in Google Scholar after posting preprints at BioRxiv and ArXiv has been described in 2014:...

29 November 2022 6,857 0 View

What post-hoc is appropiate for repeated measures ANOVA?

Dear colleagues! I have two groups of animals which I compare using 8 day long behavioral paradigm. For each day I have two data points (day and night). Thus I have one independent factor (animal...

04 August 2021 4,490 4 View

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Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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Anyone having idea about VN primer for miRNA primer design ?

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Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

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PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

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