Do you have a protocol for PCR of lentiviral genome?

06 June 2014 1 7K Report

I would like to deep-sequence lentiviral genomes. The first step would be to extract and then amplify the genome by PCR. Do I need to RT the genome into DNA before PCR? Or can ssRNA serve as a template directly? Does anyone doing that routinely have a well established protocol for me to follow? Or any experience to share?

Xinping Huang

I use qPCR lentivirus titer kit, the link is here:http://www.abmgood.com/High-Titer-Lentivirus-Calculation.html

it's easy, but a little expensive. Good luck!

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Science method

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