Atefeh Khamoushi

3 Questions 1 Answers 0 Followers

Questions related from Atefeh Khamoushi

How to make a site specific mutation in LNCRNA for luciferase assay?

Hi I should do luciferase assay to confirm the LNCRNA/miRNA interaction. I should do pcr and clone the whole sequence of lnc in a expressional cloning vector and I should also do it mutated form...

08 July 2024 443 2 View

Why my pcr bands are fuzzy?

Hi Although the ladder is sharp but my pcr bands are too fuzzy and like smears. I try to set up one of my genes but it still stands for 3 bands, unfortunately. Tm is 65 but there are still...

18 June 2024 7,861 1 View

Do we have to do all our cellular tests by treating with ic50 con?

Hi, I have done mtt test and realized that my reagent ic50 is 50nM. Do I have to do ally cellular assay by treating with this concentration? Do we do the mtt test for this purpose? Of course I...

11 May 2024 1,387 2 View