Sreelakshmi S Kumar

4 Questions 2 Answers 0 Followers

Questions related from Sreelakshmi S Kumar

Do we have to check the efficiency of sonication by doing a gel run everytime before proceeding for ChIP?

I am trying to optimize ChIP for my cell lines. I am doing with WT cells. I keep checking sonication efficiency on an agarose gel. So if I take a new sample of the same cell line, do I have to do...

07 May 2024 7,200 0 View

How to do efficient manual counting of cells?

I am struggling with manual counting using neubauer chamber. We have automated counter as well. Manual counting shows lesser cell density while automated ones show more. Mostly people say manual...

01 May 2024 164 0 View

Could anyone tell me the conditions to sonicate aml cell line samples for ChIP?

I am trying to optimize sonication for ChIP of AML cell lines viz. HL60 U937 THP1. I am fixing cells with formaldehyde and sonicating 2.5 million cells at a time. If anyone has worked with these...

01 May 2024 7,680 0 View

ChIP Sonication optimization

Hello, I am trying to optimize sonication for ChIP. I am working with aml cell lines viz. HL60 U937 and THP1. I have fixed the cells with 1% formaldehyde and stored at -80 degrees. There are two...

01 January 1970 1,154 0 View