I am conducting fecundity works for Macrobrachium rosenbergii. However, some of the eggs samples have irregularities in shape and size. Thus, this has made me a bit confuse while conducting the experiment. Refers to the picture given:
Picture 1: Represent a good sample with same shape and size.
Picture 2: Represent not a good sample with different shape and size.
There are three different conditions of eggs that I had recognized and need to be justified to include it in counting or not (Refer to Picture 2 Box A, B & C). The question is, do I need to include eggs in Box A,B or C in counting ? If yes (or not), what is the clear justification would be made in these situation?
Picture 2: Represent not a good sample with different shape and size.
What you could do is sieve the sample and count the eggs in the different portions. Could provide valuable information on variable egg sizes, % irregular or deformed eggs, etc.
Do a random sampling of the collected eggs and count the healthy/viable and deformed/dead eggs.
Go for a random sampling and count all the eggs for the estimation of fecundity as fecundity is the egg laying capacity of the animals(eggs may be viable or nonviable)
Do random sampling and count all the eggs for fecundity estimation. You can go for diameter measurement also which will provide a clear cut idea about size of viable and nonviable eggs.
Besides what previous researchers said, would count all and would make a percentage of good and bad eggs and get proportions and determine what should this
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