Can we activate the cytokine secretion in THP-1 monocytes by using the flagellin? Do we first need to differentiate monocytes into macrophages before applying flagellin?
I came across one publication mentioning about inducing THP-1 with retinoic acid to overexpress TLR5 but others did not mention about it so I am confused. Can someone please help me?
Hi Nasir,
Whether you should activate THP-1 cells with PMA and use differentiated macrophages for your study depends on your biological question as the cytokine responses vary considerably between the two. However if you are concerned only about expression of TLR5 on THP-1 cells for stimulation with flagellin, then yes, THP-1 cells do express TLR5.
THP-1 monocytes themselves express TLR5 on surface and hence there is no need for differentiation into macrophages. But, a word of caution, before doing your experiments make sure your flagellin is not contaminated with LPS. You can use the LAL assay to detect LPS in your flagellin.
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