the sample was isolated from polluted river water and cultured in f/2 media according to the European standard. The images are a little bit confusing, so can you help me to id them?
The images have too little magnification to be able to say anything for sure. With approx. 400X magnification, diatoms look like this, for example: Unidentified alga from fresh water.
Beamlak Teshome are you able to get any pictures at a higher magnification?
I think, it`s not diatoms.
You can also dry the preparation, and then you will see that these are not diatoms - the cells are wrinkled
The images are not visible enough.But the second one is Euglena viridis. The first is likely to be a diatom but not clear enough to identify the species
Hi Beamlak,
Not the best images for identification, in terms of clarity. No need for phase contrast, brightfield is fine, as is your 20x objective, for a 200x total magnification, though 400x is better.
With these images it's not clear if they're even diatoms.
Anna R. Grendys unfortunately I was not able to get higher magnification. But, i will try to find one.
does this work?
Maryna Zhezhera thank you ma'am. Do you have any suggestion on how to get rid of them in diatom cultures. I think there thermotolerant property allowed them to survive the sterilization steps.
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