Do the reduced proper motions of the stars depend on the observing condition?

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Any protocol for loading dsRNA in an agarose gel?

I am expressing dsRNA using E.coli as vector. I would like to check that my bacteria culture is expressing dsRNA. I am planning to extract RNA from my bacteria culture and then load the total RNA...

18 March 2024 1,371 3 View

Is there a method to stain ingested bacteria?

I am working with a marine non-model organism that has a planktrotrophic larval type and I found out that the larvae are not very selective while feeding. I am planning to do RNAi by feeding them...

25 January 2024 7,678 3 View

Do you have experience working with ascorbic acid?

If we treat NHDF cells with ascobic acid for 1 hour at 33ug/mL and then irradiate the cells with a low dose of UVA we see a good antioxidant response and cell viability does not change compared to...

04 October 2023 2,101 1 View

Is it possible to analyze single nuclei RNA seq libraries with an assembled transcriptome or only with the genome?

I am trying to generate single nuclei RNA seq libraries from a non-model organism. We have a genome that it is not well annotated and a bit incomplete. I know that for single cell RNA seq is...

02 October 2023 5,180 1 View

Are Alamar blue results confusing?

Hi everyone, We are recently doing cell viability and for that we use Alamar Blue. The results after ascorbic acid treatment and UVA-irradiation show increase cell viability for cells that were...

20 September 2023 681 2 View

What statistical tool should I use to determine if there is a significant relationship between curriculum enhancements and learning growth rate?

09 September 2023 9,332 7 View

Does anyone have experience coating microplates for Elisa with more than one allergen?

Hello everyone. I'm trying to run an Inhibition Elisa of two purified allergens from D. pteronnysinus at the same time. So, for this, I coat the microplate with the two allergens and then, for the...

08 June 2023 5,920 0 View

Are there any alternatives to using a peristaltic pump for a perfusion of a murine liver to extract cells?

Can the perfusion be performed with any other alternatives? Preferably a low cost option.

13 May 2023 8,276 0 View

I am a student researcher and I'm having troubling in making our situational analysis, can you please help me?

Our research title is Social Influence and self esteem towards career decision making of senior high school students. Our social influences are Family, peers, teachers and romantic relationship,...

03 July 2022 1,759 2 View

What statistical test to use?

Hello, please I need help with a statistical analysis. I don't know what statistical test to use. The study I carried out is as follows: Quantify polyphenols, flavonoids and anthocyanins in...

04 June 2022 8,843 4 View

In terms of chaos, what is the necessary and sufficient condition for authoritarianism, permanent or temporary, to come to exist and persist?

Since 2016 Brexit, the world needed to change the thinking behind traditional democracy as the democratic landscape changed, yet traditional democratic thinkers and actors have been acting as if...

28 July 2024 6,515 1 View

What could be the possible reason that sintering of polycrystalline diamond fabricated with tape casting has suddenly started failing?

I make polycrystalline diamond with tape casting followed by HPHT sintering. When i sintered the samples up till May, the sintering was good. When i sintered the samples in June and July at the...

16 July 2024 3,212 5 View

Need help is coverslipping-Histology samples?

Hello, Our lab has been experiencing sporadic issues with coverslipping. We use the Sakira Tissue Tek Prisma H&E Stainer and a Tissue Tek Glas2 Automated Glass Cover slipper. Periodically,...

12 July 2024 7,016 3 View

What is the current status of augmented learning in robotic surgery?

I would like to perform a literature review at this time on augmented learning and learning augmented algorithms to enhance performance-guided surgery

06 July 2024 246 1 View

What is the necessary and sufficient condition for normal democratic outcomes to keep power regardless of the comings and goings of exism movements?

You have seen the comings and goings now of Trumpism, Brazilianism, and Brexism, 2016 to 2024 and the common theme is why they failed to persist in power ONCE THEY CAME TO POWER. If you look at...

06 July 2024 9,022 1 View

Rethinking democracy: What are the 3 fundamental lessons learned from facing exism movements and dictatorship threats 2016-2024?

Are you concerned about the future of democracy, locally or globally? What do you think the fundamental lessons learned for democracy are since 2016 BREXIT? How can we come out with a...

06 July 2024 5,962 4 View

What is the necessary and sufficient condition for permanent dictatorships to remain in power regardless of opposing democratic movements?

You have seen the comings and goings now of Trumpism, Brazilianism, and Brexism, 2016 to 2024 and the common theme is why they failed to persist in power ONCE THEY CAME TO POWER. You have seen...

06 July 2024 6,790 1 View

Is science based on a political definition science?

It seems that since 2012 Rio +20 Conference, the world has been moving away from science based perfect market thinking, a moved that apparently has been helped by the confusion created by the 2012...

02 July 2024 4,530 4 View

5th International Conference on Advanced Engineering Technologies?

Dear Researchers, Bayburt University will organize 5th International Conference on Advanced Engineering Technologies (ICADET) on 25-27 September 2024 The event is scheduled in Hybrid Format,...

30 June 2024 1,200 0 View

What are the regulatory guidelines to submit a microorganism as Generally Regarded as Safe ?

Give in detail with available literature and website links for submissions

27 June 2024 9,753 1 View