this relates to the caogulation assay performed with synthetic kaolin solution in spectrophotometer
In general absorbance/ optical density should be less than 1. As higher absorbance value may lead to non-linear dependence of Lambert-Beer's equation. However depending on the spectrometer brand the upper limit of absorbance may be higher (please refer to the manual). In case you cannot dilute the sample please choose a cuvette with smaller path length to decrease the absorbance.
Dear Mr Arun,
Except in case of concentration dependent analysis, the value of OD should not be greater than 1 as the Beer's-Lambert law is not valid for higher concentration. As per equation A = 📷 * b * c, deviations in absorptivity coefficients takes place at high concentrations (>0.01M) due to electrostatic interactions between molecules in close proximity.
yes, because the value of OD should not be greater than 1 as the Beer's-Lambert law.
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