or GCMS, TLC are the only way to determine the monosaccharide presence
Hi Arun,
FT NMR a good technique for structural analysis of all chemicals including monosaccharides. If possible dry your samples prior to making solution in deuterated solvents. Deuterated oxide will be fine. You will get proton functional and structural groupings, very sensitive (99.978% abundance), 13C will give good spectrum of distributed 13 carbons. 135 and 90 depth are important and comparative information needed. In addition do Cosy 1Hv1H: 1Hv13C for proton carbon and carbon carbon specific bond links. GCMS to confirm.
John.
The short answer to your question is "yes".
You might want to look up some of the following articles on the Internet in order to get some more detailed information on the subject.
NMR Spectroscopy in the Study of Carbohydrates: Characterizing the Structural Complexity, William A. Bubb, Concepts In Magnetic Resonance Part A, Vol. 19A(1) 1-19 (2003.
Nuclear magnetic resonance spectroscopy of carbohydrates, from Wikipedia.
Carbon-13 Nuclear Magnetic Resonance Spectroscopy of Monosaccharides, Klaus Bock , Christian Pedersen, Advances in Carbohydrate Chemistry and Biochemistry, Volume 41, 1983, pp. 27-66.
1H and 13C NMR Shifts for Aldopyranose and Aldofuranose Monosaccharides: Conformational analysis and Solvent Dependence, Paul Hobley et al., Magnetic Resonance in Chemistry, Volume 34, Issue 10, October 1996, pp. 755-760.
Peter Norris, Ph.D., Monosaccharide NMR examples from the lab; gives links to the 400MHz 1H NMR spectra of 10 compounds.
Carbohydrate NMR-revolvy.com.
Many additional references.
Good luck in your research! AF
P.S. D2O is often used as solvent for carbohydrates. OH protons will exchange, and cannot be observed in that solvent.
Hi Arun,
As Anthony just said, the answer is yes.
To add some elements, if your goal is to identify the nature of a monosaccharide, the chemical shifts values of 13C signals are a good way to do it. You can easily find some tables on the internet. COSY and HSQC can also be very helpful for the characterization of a monosaccharide.
However, if you just want to show the presence (or not) of monosaccharide into a mixture, I think TLC can indeed be a rapid and easy way to start. There are some reagents for the specific detection of carbohydrates on TLC (such as orcinol for instance).
Pierre-André
As everyone has said already ... YES. In fact NMR is probably by far the most powerful tool for identifying carbohydrates.
You might want to look at eurocarb.db if you want to get some reference data for chemical shifts, etc. But there are many resources out there. Here's the link to Eurocarb.DB:
http://relax.organ.su.se/eurocarb/
If you just want to check for presence/absence there are many options - including simple colorimetric tests. If you want to get as much information as possible - NMR.
Hi Arun, as long as more than 5wt% of your dried sample is a specific monosaccharide you will be able to determine it with the current state of the current state of the art NMR spectrometer (given that the total sample amount is sufficient for obtaining good signals).
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