Do syber greens form different companies give the same foldchange in qPCR?

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Dianaa Londoñob

The performance of syber green is influenced by different factors including the brand of your thermocycler.

To identify if your results are correct you must consider the background of your assays and put a negative control (which could be a mix without cDNA) to manage that background and calculate your real signal in your samples and housekeeping.

Can Kiessling

You should get similar values. You can run the same sample twice with the same reagents and you won't get the same exact fold-change. Hence why you use average values and SEM.