How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

How to prepare a liquid sample for FTIR spectrum?

I have a ethanol-water 50% extract of mango peel. I purified mangiferin from the extract with filtration and precipitation. so i obtained a low concentrated extract which i want to se the IR...

24 January 2021 8,880 7 View

Can you trust vaccines that were made in a year, will help fight Sars-Cov2?

That vaccines have been produced in such a short time, makes me doubt if they will help fight this pandemic and if they will not do us more harm.

06 January 2021 3,469 19 View

What is the impact of the Christmas holiday on the spread of coronavirus?

What is the impact of the Christmas holiday on the spread of coronavirus? since some countries have already started vaccination, will we see a decrease in the rate of contamination?

26 December 2020 4,917 4 View

Should sampling and reconstruction be matched? How should the sampling be for machine learning algorithms?

In image reconstruction, we usually design reconstruction algorithms in such a way that we can choose the most convinent sampling pattern. However, the choice of the sampling pattern has a...

10 November 2020 8,669 1 View

Oocyte collection (GV and MII)?

Does anyone have a protocol for simultaneous collection of VG and MII oocytes in the mouse (from the same animal)? Thanks

06 September 2020 1,091 3 View

Insects and COVID-19: a possible contaminant?

Dear colegues, What do you think about this? Can insects or others arthropods transmit the virus (directly or indirectly)?

19 April 2020 7,391 53 View

Does anyone have experience/reference with SARS-Cov-2 detection by qPCR without extraction?

With the shortage of reagents for molecular tests worldwide we are looking for a protocol that doesn`t need to have an extraction step and that can directly detect the SARS-CoV-2 virus using a...

20 March 2020 3,479 4 View

Forests Pests: increased damage by climate change?

Dear researchers, what is your opinion about climate changes and forests pests? there is an increase or reduction of damage caused by insects and mites on trees?

29 February 2020 2,434 17 View

How to improve current in Natural DSSC?

Hello everyone! I'm struggling to improve my natural DSSC. Could anyone give me a hint? What am I doing wrong here? My parameters and characteristics below. Picture of one of my cells...

23 January 2020 1,614 9 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View