Do perfusion and fixation with paraformaldehyde 4% in PBS interfere with RNA extraction from brain tissue?

More Itztli Trejo-Sánchez's questions See All

Could someone please provide a list of journals that accept the application of methodologies in nature-based solutions?

I am looking for journals that admit the publication of results from applying the IUCN Standard for designing and monitoring Nature-based solutions. Many thanks for considering my request.

23 July 2024 6,444 2 View

About mineral exploration?

Are there any techniques at ore exploration that could replace the compass-tape measure of mineralized structures?

14 June 2024 3,254 2 View

What is the minimum amount of liquid and beads I can use for bead-beating in a 2ml vial?

I am planning to extract DNA with QIAamp Mini DNA extraction kit (Qiagen) from DNA Shield microbiome preserved samples. My samples contain small amount of cell, so my goal is to reduce the loss of...

14 May 2024 2,291 2 View

Can I replace some of the buffers from the first steps of QIAamp Mini kit by DNAshield?

I am planning to extract DNA with QIAamp Mini DNA extraction kit (Qiagen) from DNA Shield microbiome preserved samples. My samples contain small amount of cell, so my goal is to reduce the loss of...

14 May 2024 7,178 3 View

How do I measure the virulence intensity of a fungus strain in a host plant?

I need to determinate how virulent is a fungus/oomycete strain-specie in comparative with others. I have plants group with and without fungus symptoms. However I found the fungus sources in both...

06 May 2024 8,974 5 View

¿How can I publish a book chapter without payment?

I almost finished a book chapter about my resent research (modeling of solid biomass combustion using OpenFOAM) and I want to publish it. In the case of a paper the procedure consists of sending...

26 February 2024 2,352 6 View

Application of pulsed light?

What are applications of pulsed light on produce?

19 February 2024 7,686 0 View

Ranges of psychiatric evaluation scales?

What are the ranges of the scales: Social Functioning Scale (SFS): to measure social functioning BPRS (18 items) and BPRS-E (extended version, 24 items): schizophrenia symptoms PANSS:...

27 January 2024 2,960 3 View

I am trying to detect a secreted protein by Western blot, Does Any of you have experimented some problems identifying secreted proteins by WB?

I am trying to detect a secreted protein by Western blot in plan tissue. To this aim, we are using a simple protein extraction protocol based on TX-100 and DTT. Then, I am loading the samples in a...

14 January 2024 1,647 4 View

Best way to fit mosquito incidence model with future climate data?

I have tried with no avail to make a logistic regression and random forest model work. First i fitted a model with climatic measurements as independent variables (2015-2020, almost five years)...

10 January 2024 9,738 1 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

Can telekinesis, telepathy, and prediction be achieved through science?

Information in The Three Dimensions of Time.Information in The Three Dimensions of Time Information Transfer based on Brains Entanglement.Information Transfer based on Brains Entanglement Time...

28 July 2024 7,522 0 View

Why only alpha wave of brain are found in some patients?

I have found an EEG where only alpha waves are present. Beta waves are not found in active patients. What interpretations ?

26 July 2024 4,741 1 View