Does anyone have experience on maintaining the NSG strain from JAX? I found 3 genotyping protocols on the JAX website but was wondering if it is necessary to screen them on a regular basis if one's going to keep the strain?
Thanks in advance for your advice!
Hi,
You should probably screen the mice to avoid misbreading mistakes and leakage.
We are setting up a genotyping process at our institution too (for NSG, SCID and NRG mice), and have emailed with the JAX service about some of the questions involved. Here are excerpts from their replies, I hope they are helpful:
For all three strains all the listed mutations are maintained as homozygous or hemizygous (the Il2rg gene is located on the X chromosome, so mutant males are hemiozygotes).
Therefore when you genotype with the Prkdc assay, the NOD/scid (Stock# 001303) and the NSG (Stock# 005557) strains will show only the mutant allele. The NRG mice (Stock# 007799 ) will only show the wild type allele.
When genotyping with the Il2rg assay, the NSG and NRG strain will only show the mutant allele, and the NOD/scid only the wild type allele.
When genotyping with the Rag1 assay, the NRG mice will show only the mutant allele, and the NSg and NOD/Scid only the wild type allele.
good luck
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