How to avoid ATP degradation while performing ATP assay?

I am using the ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) for measuring intracellular ATP from treated cell lines. Although my standards are wokring fine when using the fluorometric...

15 February 2021 3,746 4 View

Which photsynthetic machine is good to use for measruing photysnthesis and CO2 assimilation for Arabidopsis?

Hi all, we are looking to buy a photosynthesis meter that can potentially measure photosynthetic rate, stomatal conductance, CO2 assimilation, PS1 and PSII efficiency (if possible), etc. it would...

02 February 2021 5,223 3 View

What is the relationship between Sensitivity ratio and liquidity index especially for quick clay?

Also if anyone can share latest research regarding Geo-disaster of quick clay except in Norway? Thanks Kind Regards Dr. Khan

05 January 2021 4,645 3 View

How to detect the compounds present in an aqueous extract of a plant material on tlc? And please suggest me ratio to extract?

I am doing tlc but how can i check my result. Because under UV, it is not possible to see.

31 December 2020 960 3 View

How can i describe the different bands on tlc plate, plant extract?

On tlc plate i get some colorful band under uv 360nm, like pink, brown, indigo, sky blue and some others. How can i tell that this color indicates this compound? And in which extract hexane,...

21 December 2020 3,423 3 View

How to get Argo Data? and is this useful for water column SST data?

I am working on MODIS-SST, I need SST in situ data and water column data, Please guide me in this.

21 December 2020 9,935 5 View

If we are going for co digestion of microalgae with wastewater but the sample is having low pH, What are the effective ways to increase the pH ?

wastewater with low pH

07 December 2020 2,716 3 View

Interested to publish you work related to redox-mediated signaling in plants under stress conditions?

We have launched a special issue on " Redox-mediated signaling in plants under stress conditions". Please see further details in the following...

16 November 2020 8,334 2 View

How to print increments/time steps when running a batch file in cmd window for abaqus analysis?

Hello Everyone, I created a batch file to run the multiple jobs using abaqus solver. It is working well. However, I cannot see the increments/time steps in the cmd window. It just showed "Run...

12 November 2020 9,641 3 View

How can I identify compounds from tlc plate if they give different color under Uv?

The tlc plate show pink, sky blue and purple color under uv light. is there any standard through which i can read that this color show this compound? and also there are some compounds which show...

06 October 2020 6,204 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View