Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...

17 February 2021 7,802 2 View

How can we make remote teaching and learning more interesting and engaging?

We ( Higher Education) started with Zoom, then Google Meet, and this term we will be using more updated and smarter system MS TEAM to make online teaching more participatory and engaging and...

12 August 2020 9,154 42 View

How to fix atoms with same coordinate error?

I am getting a fatal error in gromacs while creating a GRO file and ligand topology file under the amber force field. The acype of python providing the error message. Parameter was: Charge =...

29 July 2020 3,347 4 View

Correlation coefficient and Mutual information range?

what is the difference between correlation coefficient(CC) and mutual information(MI)? what is the range of both methods (CC and MI)?? I want to evaluate image registration through these two methods.

16 July 2020 2,767 4 View

Is there any way to implement the MATLAB code in the genetic algorithm which solves optimization problem of image registration?

I want to design an algorithm which solves the optimization problem in image registration through genetic algorithm and also design stationary field. Give me your suggestion for this idea is it...

14 April 2020 3,237 4 View

How to improved results of medical image registration ?

I have improved diffeomorphic log demons image registration. Now what should i do for improving results. what should i add in my registration algorithm. Can you suggest me something who are expert...

29 March 2020 2,054 3 View

How can I solve error, getting "dplyr" package for R 3.6.0 ?

15 January 2020 3,150 3 View

How to get uniform coating of graphene oxide?

Hello all, Could anyone suggest me how to get a very uniform graphene oxide film? I used dip coating, spray coating and spin coating of GO on glass slide. But none of these methods worked. When I...

23 July 2017 6,469 5 View

How to determine the latitude and longitude of a target?

I have the position of a RADAR station and some aitrcrafts' azimuth, elevation and range data. How can I calculate the latitude longitude of those aircrafts?

30 August 2016 6,636 6 View

How to find an optimum route, in a network, with minimum travel time and maximum served demand by usin Genetic Algorithm in MatLab?

I have network comprising of nodes and links, also the travel time information and demand at certain nodes ; all in the form of matrix. If i know the Origin and destination point, how can i find...

15 April 2016 5,400 2 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

Exit velocity of natural gas from pressurized tank?

Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...

26 February 2021 6,253 3 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View

Why CNN algorithm performs well in Network Intrusion Detection based on some recent resources?

Most of the papers have compared different hyperparameters such as layers, initializers, activation function, and optimizer algorithm in the CNN algorithm for detecting network intrusion. This is...

24 February 2021 4,578 4 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View