Hi Juliette! In my experience OVA solution for more than 24h became putrid and stinky and animals avoid drinking it. You can smell the bottles for yourself. I recommend you should change OVA solution every day during your oral protocol. I also use an egg white solution diluted 1:5 in water and tolerance obtained are even better, when we analyze difference of specific antibody titers between tolerant and immune mice.

i attached my article which might be useful.

Thanks a lot,

this is exactly the kind of advice I was looking for !