I am having difficulty in finding out whether or not I should activate my cells or cell supernatant in order to measure the expression of Amphiregulin using a quantitative ELISA kit.
Has anybody any experience with this, or could advise me?
Dear Anselm,
Thank you very much for the response, it is very helpful.
Kind Regards,
Elaine
Dear Anselm,
Thank you for the advice regarding the ADAM17 activation, however I wanted to ask another question.
If you have experience with this, what sort of control would you use to compare against the activated cells.
Surely the non-activated cells aren't a comparable control as one should not be able to measure the AREG shedding in the supernatant, when not activated?
Apologies if this is a very simple question, I'm not so well experienced with this type of work as of yet.
Kind Regards,
Elaine
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