Do I need glycerine to weigh down samples of DNA/ Protein?

More Micah D Langford's questions See All

Computational tool for reading amino acid sequence

Hello, I was wondering if there was a computational tool which I could use to read the amino acid sequence for PB ID: 3igm. I was considering using the FASTA format, but I received poor output...

03 April 2016 2,382 6 View

Monarch DNA Gel Extraction Kit Spin Speed?

Hello, I was wondering if anyone had experience using this kit for DNA-protein complex gel extraction. If so, how did it work out? If not, then would you forsee any problems with my using...

02 March 2016 8,743 3 View

Ethanol used for DNA/Protein Complex Extraction?

I was wondering if I was thinking about this right: My DNA-Protein Complex would break if I used the phenol-chloroform extraction which would completely mess up my experiment. So, my other option...

02 March 2016 985 2 View

Low Protein Binding Collection Tubes Thermo Fisher Protocol?

Hello, I am trying to find a good protocol for using these tubes. Does anyone know of any? I am targeting a DNA binding protein. I am new to this side of the sciences, so any help would be...

01 February 2016 9,637 2 View

Protein storage protocol and length?

I was wondering if you knew of a good protocol for storing protein for about 1-2 weeks. Also do you know how long I can keep the protein? Thanks, Micah

01 February 2016 7,227 4 View

Using Ethanol for DNA Extraction For Gel Electrophoresis?

I was wondering if I could use ethanol for DNA extraction for running a gel. Any precautions? Tips? This is the protocol I was thinking of: Pipet out the supernatant and combine pellets to...

01 February 2016 6,800 5 View

What buffer should I use for Adipocyte Protein 2?

Hello all! I am a student at CPCC and am doing a project dealing with Adipocyte Protein 2. I was able to discover the pi value and the isoelectric point. Thus giving me the pH value of the buffer...

31 December 2015 6,777 2 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View