Computational tool for reading amino acid sequence

Hello, I was wondering if there was a computational tool which I could use to read the amino acid sequence for PB ID: 3igm. I was considering using the FASTA format, but I received poor output...

03 April 2016 2,318 6 View

Monarch DNA Gel Extraction Kit Spin Speed?

Hello, I was wondering if anyone had experience using this kit for DNA-protein complex gel extraction. If so, how did it work out? If not, then would you forsee any problems with my using...

02 March 2016 8,676 3 View

Ethanol used for DNA/Protein Complex Extraction?

02 March 2016 882 2 View

Low Protein Binding Collection Tubes Thermo Fisher Protocol?

Hello, I am trying to find a good protocol for using these tubes. Does anyone know of any? I am targeting a DNA binding protein. I am new to this side of the sciences, so any help would be...

01 February 2016 9,488 2 View

Using Ethanol for DNA Extraction For Gel Electrophoresis?

01 February 2016 6,669 5 View

Protein storage protocol and length?

I was wondering if you knew of a good protocol for storing protein for about 1-2 weeks. Also do you know how long I can keep the protein? Thanks, Micah

01 February 2016 7,137 4 View

What buffer should I use for Adipocyte Protein 2?

Hello all! I am a student at CPCC and am doing a project dealing with Adipocyte Protein 2. I was able to discover the pi value and the isoelectric point. Thus giving me the pH value of the buffer...

31 December 2015 6,705 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View