I woultd like to study the microbial diversity of hypersaline water by PCR-DGEE followed by sequencing. Before this step I have sequenced the 16s rRNA gene of some isolated strains from this sample by using the following archaea specif pair of primers
A20F (TCC GGT TGA TCC TGC CG)
A1540R (GGA GGT GAT CCA GCC G)
Do I have to use this pair of primers in DGGE?
For the DGGE analysis of bacterial diversity I will use another pair of primers.
Hello, I used the following universal primer set for total biodiversity of bacteria.
Forward Primer
27F (5’-AGAGTTTGATCCTGGCTCAG-3’)
Reverse primer
1492R (5’-GGTTACCTTGTTACGACTT-3’)
Good luck!
Forward NL1 5’ GCCATATCAATAAGCGGAGGAAAAG-3’
Reverse NL4 5’-GGTCCGTGTTTCAAGACGC-3’
These universal primers for yeasts.
You can use the following universal primer set for total biodiversity of halophilic bacteria.
FD1 5’-AGAGTTTGATCCTGGCTCAG-3’
rP1 5’-ACGGTACCTTGTTACGACTT-3’
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