I intend to produce viral particles containing hTERT protein and SV40Tantigen for cell immortalization of my primary epithelial cells using plasmids such as pBABE-puro-sv40T. I am searching for packaging cell line. I want to know if HEK 293T per se can be used or should I go for specialized cell lines?
Also, if I intend to use HEK 293T cells, must i procure viral genes containing plasmid?