Do HEK 293T cells contain gag-env-pol genes to make viral particles?

12 April 2014 0 695 Report

I intend to produce viral particles containing hTERT protein and SV40Tantigen for cell immortalization of my primary epithelial cells using plasmids such as pBABE-puro-sv40T. I am searching for packaging cell line. I want to know if HEK 293T per se can be used or should I go for specialized cell lines?

Also, if I intend to use HEK 293T cells, must i procure viral genes containing plasmid?

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