Do DNA strand breaks always result in damage? How?
No because some mutations are necessary for individualized human survival. Therefore, breaking DNA strands does not always damage, and by extension, age a human individual.
Hi I am trying to model a cutting problem with a blade and comparing the results with and without ultrasonic vibration. From experimental results in our group, the use of ultrasound can...
13 June 2024 7,461 4 View
I'm conducting research on photoplethysmography (PPG) signals obtained from smartwatches worn on the wrist. The main goal is to analyze the PPG waveform and extract key fiducial points and...
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I am running some simulations in Abaqus to extract the stiffness matrix of a FE model with geometric nonlinearity. I want to provide to Abaqus a set of nodal displacements (all the dofs in the...
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Hello together! I have some time-series for tree-rings from dating from 1850-2016 and diameter at breast height (DBH) from 1996-2022 for the same plot, however the sampled trees for tree-rings...
16 May 2024 5,295 2 View
I think so because The Random Slate Theorem
03 May 2024 3,082 7 View
I am currently trying to get a Skyscan 1072 micro CT to work again. After the mainboard on the original PC died, we tried to set the machine up on a new computer. We managed to control the source,...
28 April 2024 381 0 View
Is it worth using a paired t-test on pre and post survey data with 12 or less responses? Or should one rely more on the mean and standard deviation of the two data sets to evaluate the survey...
27 April 2024 8,703 8 View
1)Religion(or at least spirituality) calms my mind, particularly, the built in belief of only one reality. God created the only reality. The afterlife is another dimension. We have free will but,...
25 April 2024 10,055 0 View
I have 217 preprocessed BrainVision matlab files (.mat) with ECG data in them. I've been having trouble creating a study for these. I know BioSig works for ECG data, but it doesn't seem to work...
21 April 2024 1,028 2 View
I have 25 folders for 25 participants, all with .mat files inside that are preprocessed. Most of them have the same amount of .mat files - a couple participants didn't finish the study, so they...
17 April 2024 1,897 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
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I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Details of the Analysis. Static Analysis Composite Layup Continuum Shell Elements FRP Material (Elastic and Hashin Damage)
03 August 2024 8,538 4 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Sure, here is the translation: I have a 3D orthogonal woven composite structure where warp, weft, and binder yarns are oriented in three directions. After modeling these yarns and the matrix in...
28 July 2024 8,169 0 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View