I made some competent cells, but using those cells for molecular cloning will produce a lot of wrong plasmids, and the colonies grow very few, so will competent cells affect molecular cloning to construct plasmids?
According to my knowledge, plasmids have been constructed before the transformation process so those can not be altered during the transformation. You are getting the wrong plasmids, it might happen due to some kind of contamination. You are getting fewer colonies which means the competency of the cells is not good and you might allow cells to grow for long to get colonies and which causes contamination. Prolonged incubation to get colonies is not good practice. You can use alternate methods to prepare competent cells like ultra-competent cells or electroporation.
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