Do anyone have a mammalian expression vector which includes N- or C-term 6xHis tag?

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Cigdem Ozen

Dear Hizlan,

which pcDNA do you want to use? Just give a call on Monday, ok?

Cheers

Huanting Liu

If you already has an expression vector in hand, the easier way get your work done is to order a pair of your gene specific primers containing your cloning restriction enzyme sites and one of them containing 6xhis coding sequence (either at N-terminus, his coding sequence in 5' end primer or C-terminus, his coding sequence in 3'end primer), PCR amplify your gene, digest your PCR product with the designated R/E and ligated it into the same R/E digested vector.