Hizlan Hincal Agus

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Questions related from Hizlan Hincal Agus

Do anyone have a mammalian expression vector which includes N- or C-term 6xHis tag?

I would like to synthesize recombinant target protein in mammalian cell lines. Can anyone lend me a 6xHistag expression plasmid, such as pcDNA with 6xHistag.

08 August 2015 8,298 2 View

Co-Immunoprecipitation problem - Can anyone lend their expertise?

I want to purify a protein complex with co-IP kit (Thermo-Pierce, resin column), but, at the end, I could neither elute enough specific protein nor visualise by WB using antibody against...

08 August 2014 739 7 View