Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View

Not able to charge-discharge Li-ion coin cell at higher C rate ?

I am working on cathode material. I have made different Li-ion coin cell against Li metal using commercial available cathode materials such as LCO,LMO, LFP and NMC and also with the lab...

18 February 2021 1,450 3 View

How to estimate the error in the measured flow field by S-PIV?

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12 January 2021 8,271 3 View

Is it any standard method to preparation of active layer materials (blend donor & acceptor ) for organic bulk heterojunction solar cell ?

Hello friends , when I prepare blend donor and acceptor for active layer of organic solar cell I am take donor and acceptor in 1:1 ratio and concentration variation between 10mg/ml to 16mg/ml. I...

16 December 2020 3,876 4 View

How should I design electrodes for electrical stimulation on hydrogel ?

I would like to design custom setup for delivering electrical stimulation to cells cultured on electroconductive hydrogels. I am thinking to use standard tissue culture well plates (12 or 6 well...

26 October 2020 1,099 2 View

Can reduced particle size increase solubility ?

I'm trying to make Nanostructured lipid carriers (NLC) using ultrasonicator method for cancer meds that are hydrophobic and insoluble in water. I'm heating solid and liquid lipid phase and then...

21 October 2020 9,081 5 View

Please suggest any user friendly pipelines for the computation of Polygenic Risk Scores (PRS) on Microarray datasets?

I want to analyze the PRS scores for my microarray datasets, but want to use a platform which is a bit user friendly. Please suggest for any pipelines available.

06 October 2020 5,691 4 View

What is Zero air generator?

I want to purify NOx gas. Reported data shows that to achieve adsorption desorption equilibrium first we have to mix both gases.

05 October 2020 6,020 3 View

How to prepare nanoemulsion at home without a ultrasonic probe sonicator ?

I am trying to make a nano-emulsion to convert a cancer medicine into its more bioavailable form following guidance from this patent https://patents.google.com/patent/US9259391B2/en But I made...

17 September 2020 9,192 1 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

Trouble shooting with his tagged protein expression in E.Coli?

i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions

15 February 2021 8,939 3 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

Recommendations on CO2 Resistant Rocking Shaker?

A certain protocol we have developed requires the use of a Rocker/Rocking Shaker in which our dishes with cells are maintained in the incubator. The rocking is essential to the protocol but we...

09 February 2021 3,291 1 View