Do Agrobacterium tumefaciens strains LBA4404 and GV3101 require any helper plasmid if pGreen is used as a binary vector?

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Kamal Kumar

Yes, you do require pSOUP with pGreen vectors as it provides replication functions

in trans for pGreen.

Yuan-Yeu Yau

By the way, if you want to use pSOUP and pGreen set for genetic transformation, please see more discussion of the same subject from another RG member's post at below links for more useful info:

https://www.researchgate.net/post/Does_anyone_have_experience_with_the_pSOUP_and_pGreen_vector_set

Santosh B Satbhai

Generally pSoup is require with pGreen Vectors which acts as a helper plasmid...all the best

P.T. Serivichyaswat

Did you end up using pGreen/pSOUP in GV3101?