people recommend using a something called pSoup along with pGreen vectors when pGreen is used in A. tumefaciens.
Yes, you do require pSOUP with pGreen vectors as it provides replication functions
in trans for pGreen.
By the way, if you want to use pSOUP and pGreen set for genetic transformation, please see more discussion of the same subject from another RG member's post at below links for more useful info:
https://www.researchgate.net/post/Does_anyone_have_experience_with_the_pSOUP_and_pGreen_vector_set
Generally pSoup is require with pGreen Vectors which acts as a helper plasmid...all the best
Did you end up using pGreen/pSOUP in GV3101?
02 March 2021 7,082 3 View
I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...
01 March 2021 1,587 6 View
I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...
27 February 2021 4,703 1 View
It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..
26 February 2021 3,533 1 View
Greetings to all of You! I have searched some research papers for the designing of a proximity coupled patch antenna. Researchers are talking about the impedance bandwidth of the antenna but...
24 February 2021 7,662 3 View
This insect is minute in size around 1 mm and not clearly visible with naked eye.
22 February 2021 5,907 11 View
Dear colleagues, I am currently teaching myself in multilevel analysis using educational data. I found few to zero literature on basic analysis methods. In my context we frequently use level-1...
17 February 2021 9,378 5 View
Hi everyone, I would really appreciate it if someone can tell me when and why a raw fluorescence starts below zero. In the picture attached below the blue colour curve starts from a negative...
09 February 2021 4,642 3 View
I have synthesized LiMgF3 using the starting precursors LiF and MgF2 via solid state diffusion method. Now I am looking for some characterization to claim the desired product, as there is no...
09 February 2021 921 3 View
Hi everyone, I am looking for fluorescence expression vectors for expression in gram-positive bacteria present in the skin microbiome. I need to tag them with a fluorescence marker and observe...
06 February 2021 2,566 4 View
I am searching for a good place for the Post Doc,
02 March 2021 4,053 3 View
I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...
01 March 2021 7,675 3 View
Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...
01 March 2021 1,133 1 View
Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...
28 February 2021 7,356 8 View
The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.
28 February 2021 9,464 2 View
Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...
28 February 2021 9,138 2 View
What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?
23 February 2021 1,832 4 View
22 February 2021 9,932 11 View
Covid 19 Pandemic and digital transformation
22 February 2021 3,351 5 View
Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...
18 February 2021 6,500 3 View