7 Questions 12 Answers 0 Followers
Questions related from Satpal Singh
During thermal cycling, primers having self complementarity often tend to form primer dimers which usually renders the PCR reaction inefficient owing to the preferential amplification of shorter...
17 January 2017 9,214 10 View
The DNA could be either PCR generated or a product of RE digestion. Also, if there is any particular minimum limit to the length of the target DNA to be self-ligated? Thanks.
02 May 2016 7,216 3 View
also, it will be great to know if multiply fungal expression vectors with a good choice of promoters (such as constitutive and inducible) and reporter genes (such as GFP, RFP, LUC, etc) are...
02 May 2016 5,735 2 View
If we plan an experiment to study the beta-tubulin of an organism w.r.t. its capacity to interact with tubulin binding drugs like Taxol, most studies have used the tubulin purified from some kind...
16 April 2016 9,700 4 View
In particular, my target is Taxus spp. So far, i have tried Sigma kit as well as TriReagent Methods but unsuccessful. Have seen some literature also but the protocols there seemed a bit complicated.
05 March 2016 6,364 8 View
Beating with sand/beads in a motorized vessel containing buffer looks much simpler to me to deal with several hundred grams of mycelium at once.
06 May 2015 6,063 3 View
people recommend using a something called pSoup along with pGreen vectors when pGreen is used in A. tumefaciens.
10 April 2015 1,084 4 View
