DNS reagent was prepared accordingly as prescribed in Miller et al., 1959. but it precipitates when added to a sample which is acid treated. could anyone explain? is there any other alternative?
Can an MD simulation be performed by adding other salts by varying their concentration inside the box?
01 May 2023 9,230 3 View
I have stored the cm5 chip in 50 mL of HBS-EP buffer pH 7.0 at 4°C... which buffer can be used for long-term storage of the chip?
10 April 2023 4,319 2 View
MD simulation of a protein complex with more than 600 AA residues shows an average rmsd between 1-1.5 nm. can the protein complex can be considered stable? Besides sometimes i see a jump in the...
06 November 2022 6,302 4 View
I have been using CLUSPro 2.0 for protein-protein docking, but it does not take the metal ion into consideration because it selectively takes the protein chain. In contrast, HADDOCK considers the...
06 June 2022 3,285 4 View
I have done MD simulation and want to interpret and plot the data from the files. As my PC did not have enough specs for Dual boot OS because the XmGrace tool is supported only on Linux. Though, I...
25 April 2022 4,254 3 View
I am devising a study to evaluate the invitro anti- resorptive effect of my sample in bovine bone procured from butcher shops/markets. Though there are studies in which the samples from...
20 September 2021 4,259 6 View
As i am working on protein-ligand docking, i want to input the x,y,z coordinates in autodock and to do that, i need the coordinates of aminoacids present in the binding site. So, PLease help me out.
03 January 2021 4,099 4 View
Distance learning is a learning method in which students are not required to be physically present in a classroom setting or school.
25 December 2020 8,704 10 View
Some of the problems faced by farmers..... 1. Poor marketing channel 2. Farmers not getting remunerate price for the own produce 3. Lack of bargaining power (someone fix the price) 4. Monsoon...
30 November 2020 3,819 4 View
Based on my perception, organic farming we can use organic based fertilizers like biofertilizers, Azolla, Panchakavya, Neem oil extract etc. Whereas, in natural farming no application of any kind...
23 November 2020 7,080 22 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...
02 August 2024 7,843 0 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...
02 August 2024 8,572 0 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...
31 July 2024 8,309 0 View
Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...
30 July 2024 6,794 0 View
I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...
28 July 2024 8,027 3 View
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24 July 2024 3,087 3 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
Sulfuric acid has changed color to yellow.
16 July 2024 6,964 0 View