Importance of distance education?

Distance learning is a learning method in which students are not required to be physically present in a classroom setting or school.

25 December 2020 8,494 10 View

Difference on natural farming and organic farming???

Based on my perception, organic farming we can use organic based fertilizers like biofertilizers, Azolla, Panchakavya, Neem oil extract etc. Whereas, in natural farming no application of any kind...

23 November 2020 6,890 22 View

What is the exact sequence for Lac Z alpha gene including promoter/operator/terminator?

Dear Sir/Mam, I am constructing vector/plasmid with Lac Z alpha gene. I have doubt the following sequence is correct to insert Lac Zalpha-...

30 March 2018 1,075 1 View

How to solve mixed level design in Minitab 17?

To Solve mixed level design with 3 factors and factor 1(6 level), factor 2(5level), factor3(4-level), I have used Minitab general design Full factorial -with 2 replications, totally 240...

01 February 2017 9,282 9 View

What is the best way to calibrate an XRF? Use Certified reference materials as Primary standards or use secondary standards ?

What is the best way to calibrate XRF which will be used to test chemistry of Clays and Feldspars? Is going for Certified Reference Materials from companies like Fisher Scientic, MBH Analytical...

19 June 2016 3,058 2 View

How to quantify results in HPLC UV detector or any formula applying?

How can quantify in the results of HPLC per ml. e.x result 10 mg/ml.or any formula apply to quantify compound.?

13 April 2016 698 3 View

I am new to molecular works - I am getting more non-specific bands in my PCR product, how can I prevent this?

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05 March 2013 8,664 19 View

Cardiac isoenzyme expression by SDS-PAGE

I need a help, please any one suggest the procedure for the electrophoretic separation of creatine kinase isoenzymes in serum.

05 September 2011 3,973 1 View

Which tool is good for to draw the phylogeny using morphological characters?

01 January 1970 3,845 7 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

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22 February 2021 9,932 11 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Which transfection reagent is the best for plasmid transfection of MDA-MB-231 cell line, Lipofectamine based or other reagent suggestion?

I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...

19 February 2021 828 6 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View