Hi,
I am extracting mitochondrial RNA using phenol chloroform. Unfortunately one of my primers can't be exon spanning (1 exon), so I have to deal with potential DNA contamination. How can I add in a DNase treatment step? From what I've read I can add it to the RNA at the very end of my first RNA extraction, then do a second extraction again to purify the RNA. This seems like a huge pain - is it really the best way?