Shayan Singh Parihar

3 Questions 8 Answers 0 Followers

Questions related from Shayan Singh Parihar

Can I screen for auxotrophic and dominant markers in Yeast (S. cerevisiae) simultaneously on the same plate?

So if my yeast strain has a KanMX marker and also has Leu2 post transformation, can I screen both Auxotrophic and dominant markers at the same time on the same plate ex Sc- Leu with G418? If it...

24 April 2025 7,756 3 View

How to regenerate Gel Elution column for reuse?

I am using Thermo Scientific GeneJET gel extraction kit, we have no columns left but have additional reagents, now how can I regenerate them? I have read about the 1M HCl and 1M Phosphoric acid...

03 February 2025 1,076 2 View

Yeast transformation and tagging of genes by Auxin inducible degron?

I have to work on meiosis in the diploid yeast strain SK1. I have 1 gene which I want to tag with the AID cassette and study the effect of its depletion. I have amplified and purified the AID...

13 January 2025 7,777 0 View