Valerie Smaliy

3 Questions 1 Answers 0 Followers

Questions related from Valerie Smaliy

DNase treatment in Phenol:Chloroform RNA Extraction?

Hi, I am extracting mitochondrial RNA using phenol chloroform. Unfortunately one of my primers can't be exon spanning (1 exon), so I have to deal with potential DNA contamination. How can I add in...

06 December 2024 4,594 3 View

CatRAPID not working?

Hi, I'm trying to find rna binding partners through catRAPID. However, whenever I use it, instead of my result I get "Unauthorised access - please contact administrator with current URL to restore...

30 October 2023 718 0 View

In vitro transcription, changing NTP molarity, don't know how many ul to add???

So I have 4NTPs that come in 100mM, they're suggested to be used at 25mM in a solution of equal volumes and 20ul of this mixture will be used in the reaction. This is fine, however I need to...

31 October 2022 2,079 1 View