Hi Harivansh,
In the first paper below, the authors speculated that this phenomenon is due to "hydhydration water spine in the minor groove of (dA),,.(dT), sequences is greatly
responsible for the unusual B'-conformation and enhanced stability of duplex." (p.10814).
This was confirmed and more specifically detailed later by Jeffrey et al. (second paper below). "Evidence for significant hydration in both grooves of the DNA·RNA
hybrids and the DNA duplex was found in ROESY and NOESY experiments. On average, the minor groove of the DNA·RNA hybrids showed more kinetically significant hydration than the DNA, which can be attributed to the hydrophilic lining of hydroxyl groups in RNA."
1. Relative Thermodynamic Stability of DNA, RNA, and DNA:RNA Hybrid Duplexes: Relationship with Base Composition and Structure
Elena A. Lesnik and Susan M. Freier
Biochemistry 1995 34 (34), 10807-10815
2. The orientation and dynamics of the C2′-OH and hydration of RNA and DNA·RNA hybrids
Jeffrey I. Gyi, Andrew N. Lane, Graeme L. Conn, and Tom Brown
Nucl. Acids Res. (1998) 26(13): 3104-3110 doi:10.1093/nar/26.13.3104
there might be more recent papers, but I think you´ll get your answer with these 2 papers.
greetz
Hi Harivansh,
In the first paper below, the authors speculated that this phenomenon is due to "hydhydration water spine in the minor groove of (dA),,.(dT), sequences is greatly
responsible for the unusual B'-conformation and enhanced stability of duplex." (p.10814).
This was confirmed and more specifically detailed later by Jeffrey et al. (second paper below). "Evidence for significant hydration in both grooves of the DNA·RNA
hybrids and the DNA duplex was found in ROESY and NOESY experiments. On average, the minor groove of the DNA·RNA hybrids showed more kinetically significant hydration than the DNA, which can be attributed to the hydrophilic lining of hydroxyl groups in RNA."
1. Relative Thermodynamic Stability of DNA, RNA, and DNA:RNA Hybrid Duplexes: Relationship with Base Composition and Structure
Elena A. Lesnik and Susan M. Freier
Biochemistry 1995 34 (34), 10807-10815
2. The orientation and dynamics of the C2′-OH and hydration of RNA and DNA·RNA hybrids
Jeffrey I. Gyi, Andrew N. Lane, Graeme L. Conn, and Tom Brown
Nucl. Acids Res. (1998) 26(13): 3104-3110 doi:10.1093/nar/26.13.3104
there might be more recent papers, but I think you´ll get your answer with these 2 papers.
greetz
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