Is there any method which do not requires DNA modification (adding thiol etc) for attaching DNA with gold nanoparticle. I am searching a protocol for DNA attachment but as far as I know it requires DNA to be modified (I can modify gold surface)
As far as you have for example poly-A end of your DNA, you can follow some protocols. Otherwise you can modify AuNPs with some short thiolated DNA, partially complmentary to your DNA and then bind your DNA.
Attaching DNA to AuNP without any treatment is not so effective. You will only achieve non stable DNA@AuNPs because of the nonspecific interactions. DNA without thiol binds to gold via backbone or N+ from the bases, but these bonds are realy weak.
As far as you have for example poly-A end of your DNA, you can follow some protocols. Otherwise you can modify AuNPs with some short thiolated DNA, partially complmentary to your DNA and then bind your DNA.
Attaching DNA to AuNP without any treatment is not so effective. You will only achieve non stable DNA@AuNPs because of the nonspecific interactions. DNA without thiol binds to gold via backbone or N+ from the bases, but these bonds are realy weak.
Dear Varun
Attached please find an extensive review of the coupling procedures.
The best and most specific are where you modify the DNA at one end to selectively bind the DNA using one of the several now conjugation chemistries
Best wishes
Ravi
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology